Cell Biology

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The Cell is the basic unit of life.

All animals are composed of cells.

All cells originate from pre-existing cells.

All cells exist in an aqueous (fluid) environment.

Eukaryotic (animal) cells may have originated through symbiotic
associations of prokaryotic cells.

Our primordial unicellular eukaryotic ancestor presumably
lived in a salty, nutrient rich, watery environment capable
of supplying all that it needed for survival

[see Figures of exchanges between cells and their environment]

[see Fig. 1-1]

HOMEOSTASIS

HOMEOSTASIS (the tendency to maintain a relatively constant
internal environment) is, arguably, the most important and
central concept in all of physiology.

Although he did not coin the word, the concept was
developed by Claude Bernard.

"All the vital mechanisms, varied as they are, have only one
object, that of preserving constant the conditions of life in
the internal environment" Claude Bernard, 1876

Homeostasis requires a control system of some sort.

The body must be able to detect a change in the environment
and to make the needed compensatory response. Homeostatic
mechanisms monitor the INTERNAL ENVIRONMENT.

They are called closed loop systems and they depend on
NEGATIVE FEEDBACK (i.e. the deviation produces a corrective
response in the opposite direction).

[see Fig. 1-4 and 1-5]

[see Exchanges of matter figure]

GENERAL FUNCTIONS
Enzyme synthesis
Synthesis of proteins, lipids, carbohydrates, etc.
ATP production
Cellular respiration
Ionic regulation
Reproduction (most, but not all, cells)
etc.

SPECIALIZED FUNCTIONS
Gas transport (erythrocytes)
Contraction (muscle cells)
Energy storage (fat cells)
Hormone production (endocrine cells)
Transmission of action potentials (nerve cells)
etc.

WATER is the major component of a cell
(and of an entire organism).

More than 99% of the molecules in our body
are water molecules!

The human body averages about 60% water by weight
(about 70 - 74% fat-free weight)

Females, on average, has a slightly lower water content
than do males.
Water content decreases with increasing age

[see Figure of water distribution]
[see Figure 1-2]
[see text Figures 3-4, 3-5, 3-6A
[see Figure of classic (Danielli-Davson) model

Phospholipids are amphipathic (i.e. thay have a hydrophilic
[polar or ionized] group at one end and a hydrophobic
[nonpolar] group at the other).
NOTE: There are about 10 billion lipid molecules in the membrane
of a typical cell: Many, if not most, of these are phospholipids

[see Figure]
[see Figures of Fluid Mosaic model]

[see Fig. 3-9]

The plasma membrane performs a number of essential functions:
1. It regulates the movements of substances into and out of cells
2. Specialized membrane proteins (receptors) can recognize
and respond to specific chemical messengers at the cell surface
3. Specialized regions or structures can link adjacent cells
4. By anchoring various intra- and extracellular proteins,
membranes can contribute to the generation and transmission
of force

[see Figs. 3-7, 4-5 a,b,c, 4-7]

MEMBRANES

Biological membranes have been viewed for a long time as
relatively passive components of living cells.

Recent studies suggest, however, that by virtue of their acyl chain
composition, membrane bilayers may significantly impact the
thermal biology, the metabolic rate, and even the aging process
of animals.

As the term "Fluid-Mosaic Model" suggests, the plasma membrane
is a fluid rather than a rigid structure.
In order for membranes to function properly, they must be neither
too fluid nor too rigid

HOMEOVISCOSITY = the tendency to maintain a relatively constant
fluidity (viscosity) of the plasma membrane

Cholesterol contributes to membrane fluidity by blocking closs
association of the long-chain fatty acids.

Cholesterol actually binds weakly to adjacent phospholipid
molecules, which makes the lipid bilayer stronger, but markedly
less fluid,

There are two other important factors, however: temperature
and the composition of the fatty acid chains.

The fluidity of lipids is strongly dependent upon temperature.
As temperature increases, lipids become more fluid
(i.e. less viscous).

Humans, like other mammals (and birds) are HOMEOTHERMIC
(i.e. we maintain a relatively constant internal temperature).
Therefore, temperature-induced changes in membrane fluidity
are not of major importance. However, most animals are
POIKILOTHERMIC (i.e. their body temperature fluctuates
with the temperature of their environment). Some animals
live in very hot and others in very cold environments.
Many animals experience large seasonal fluctuations in
environmental (and hence body) temperature.

In light of these large fluctuations, how can animals maintain
the appropriate membrane viscosity?

Changing cholesterol content is one way (more cholesterol =
less fluid). However, the composition of the fatty acid
chains of the phospholipids is also very important.

At any given temperature, unsaturated fatty acids are more
fluid than are chemically similar saturated fatty acids.

[see figure of fatty acids]
[see figure of HOMEOVISCOUS ADAPTATION]

Cellular metabolism is strongly dependent on membrane
associated processes. This has given rise to the MEMBRANE
PACEMAKER theory of metabolism. This theory suggests that
metabolism is strongly dependent on the physical properties of
specific acyl chains.

Omega-3 polyunsaturated docosahaenoic acid (DHA) appears to
be a particularly important membrane bilayer constituent.
[Incidentally, DHA is one of the major lipids in fish oil]

[see Figure of DHA & metabolism in relation to body mass]

The current view suggests that death from old age is the
result of accumulated damage from reactive oxygen species.

Long chain polyunsaturated acyl molecules (DHA and others) are
very susceptible to oxidative attack (i.e. they have a high
peroxizability index).

[see Figure of longevity vs. peroxidizability]

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